Momoko on Ojamajo Doremi is nearly a native English speaker, and learned Japanese shortly after being introduced as a character.What he actually SAYS, though, ventures wholeheartedly into Gratuitous English territory. It probably helps that his VA is American, and also that he doesn't have many lines in the anime. Angel Beats! TK can speak perfect English.Then again, this one probably shouldn't be "surprisingly", since her full name is Aimee Blackschleger and she is an American singer who moved to Japan for her career. The song, "Future in my Hands" by Aimee B, is surprisingly well spoken English. The Devil May Cry anime series has an episode about a ghost haunting people that played her Rock records.Steer from Gundam: Reconguista in G became a fan favorite (in spite of not having a role beyond, well, steering the ship) for the way she announced her actions in loud, well-pronounced, usually singular English words.GUNDAM." in both OPs by what sounds like a deep-voiced Brit. ∀ Gundam is famous for the way the series title is announced as "TURN A.Likewise, Episode 18 shows the Renato brothers reading message board posts praising Meijin Kawaguchi III, one of which contains the Pun "Is he amazing because he's A-Meijin?". To give just two examples: Episode 8 has Nils Neilsen research his upcoming opponent Greco Logan online, and can be seen reading a fully-written article about Greco's rivalry with Italian champ Ricardo Fellini. Gundam Build Fighters contains a surprising amount of accurate English text. ![]() This should probably come as no surprise since the singer of both songs, Rie fu, spent four years in America and speaks English fluently. Mobile Suit Gundam SEED Destiny and Bleach both feature ending themes (the third and first, respectively) which transition from Japanese to grammatically-correct English flawlessly. ![]() For example, just listen to Allelujah in Episode 6 of Season 2 say "I have control".
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![]() This swivelling stylish lounge chair is available in red Leather and is extremely popular on stage sets, private events, product launches & exhibitions alike. Arne Jacobsen Children chairs evokes a calming emotion, instantly inviting your children to sit down and unwind with their favourite book. This Red Swan chair is ideal for creating a relaxed and comfortable atmosphere. As elegant as its namesake, the Matt Blatt Arne Jacobsen Swan Chair Replica (Red) has achieved cult status in the design world thanks to its enduring. ![]() When seeing the chair, you can easily imagine the silhouette of a swan on water, with it's wings folded, at peace with the world. Both chairs celebrate the curves of nature and perfectly complement one another. In our case, we have chosen to invest the 2 portion into a Stablecoin portfolio. The story behind the Swan Chair for ChildrenĪrne Jacobsen created both the Swan Chair and Arne Jacobsen Egg Chair in 1958 for the Royal Hotel in Copenhagen. To maintain our tokens’ compliance as securities exempt from registration under Regulation D and simultaneously managed by an RIA (the Red Swan Real Estate Fund LLC), a small amount of the proceeds from an offering must be invested in an unrelated asset. It is soft and pliable with a texture that caresses the hand as it runs over its surface and a fine example of its outstanding workmanship. And with it available in a wide range of colours and finishes, it is a delight for both comfort-loving kids and style-conscious parents. Egg Arne Jacobsen Style Red Swan Chair by LeisureMod This highly accurate reproduction of the original Swan Chair features the finest in materials, with no artificial materials applied to the top layer. ![]() Crafted to the same proportions as the adult version its simple form and function make it the perfect spot for your little one to curl up and fall asleep, or simply chill out in front of the TV in style. Built to two-thirds the scale of the seminal Arne Jacobsen original, the Swan Chair for Kids is the ideal addition to any stylish kid's room or fun play area. ![]() Soggiorno La Coccinella Bed & Breakfast is approximately a 15-minute drive from Peretola airport. The hotel is 800 metres to SITA Auto Stazione bus station. The location of the 2-star bed & breakfast will allow you to be within 2.4 km from the medieval Cathedral of Santa Maria del Fiore. The centre of Florence is reachable within 15 minutes' walk. Kermes is com- ferrugineus of Fabricius the Coccinella septempunc posed. This elegant Soggiorno La Coccinella Bed & Breakfast is merely a 10-minute walk from Fortezza da Basso Convention Center. published at Florence 1794, so partially precipitated by refrigeration. We propose retaining the taxonomic distinction of the North African and Canarian. Not only in their fine leather jackets and furs, but in their customer service and overall shopping experience. Coccinella algerica Ková, 1977, is synonymized with Coccinella septempunctata Linnaeus, 1758, syn. This one is not the cheapest, but it is the BEST quality. ![]() Sample Italian and Mediterranean specialities at Time Caffe located 5 minutes by foot from the accommodation. 16 reviews of David 2 Leather 'Florence is saturated with leather shops. Guests at the property can enjoy different dining options available at the lounge bar. This Florence hotel offers on-site breakfast in the restaurant. They are appointed with a work desk, and also feature coffee/tea making facilities, iron and ironing board. cc-coccinella: Roberto Ugolini Bespoke Punched Cap Toe Oxford Trunk Show. There are air conditioned rooms at the accommodation, some of them come with complimentary wireless internet and include private bathrooms fitted with a bath. Hidetaka Fukaya, Il Micio - Florence Lace Up Shoes, Mens Shoes, Dress Shoes. The Outlet is located about 30 km from Florence and can be reached easily by train or car or by bus in about half an hour. Discover Coccinelle Bags Collection and explore a huge selection of colors and style, from soft leather shoulder bag and colorful bucket bag to backpack and. Located in Fortezza da Basso district, the elegant Soggiorno La Coccinella Bed & Breakfast Florence is next-door to Porta al Prato and 20 minutes on foot from Basilica of Santa Maria Novella. Coccinelle Vintage Leather Brown Croc Embossed Crossbody Small Canteen Bag. ![]() So, with all that out of the way, let’s get started. And if you read some of those notes and emails that Laird has published, you can see that it was often an uphill battle to prevent them from making a terrible cartoon. 4Kids was a company that made nothing but s-t… EXCEPT THIS ONE TIME. I just wanted to reassure anyone reflexively gagging at the sound of “4Kids” that TMNT is the jewel in their otherwise turdly collection. ![]() Laird has posted much of his notes to the writers on his TMNT blog, and while his candor can only charitably be described as “brutal”, it’s clear that he was fiercely dedicated to making a show that didn’t suck, challenging 4Kids every step of the way.Īnyhow, this intro’s long enough. He WANTED it to be good as a matter of pride and professional integrity he wasn’t going to passively glance from the back seat like he did with the 1987 cartoon. Laird was SERIOUSLY invested in this show, not just financially, but creatively. Yes, the co-creator of the Teenage Mutant Ninja Turtles himself. The animation in this TMNT cartoon is the polar opposite of what we saw in the ‘80s: Good and consistent from episode-to-episode.įinally, the last ingredient that worked to make this incarnation of the TMNT awesome in spite of the production company heading it up: Peter Freakin’ Laird. Dong Woo was the first South Korean studio to buck that reputation by offering competent, consistent and QUALITY animation, doing shows like Men in Black, the 2002 He-Man and the Masters of the Universe and Jackie Chan Adventures. If you go back and watch an ‘80s cartoon where the animation studios would bounce back and forth between Japan and Korea, you could always tell which episode was done by a Korean studio because it would have the unmistakable thumbprint of looking like utter trash (I’m looking at you, AKOM). ![]() It used to be that South Korean animation was guaranteed to be lousy. Then you have the animation, done in South Korea by one of my favorite studios: Dong Woo. You hire good writers and see what happens? She often goes toe-to-toe with Andrea Romano as the best voice director in the American animation industry and her work includes series like Beast Wars, Men in Black and… the 1987 TMNT cartoon! So even though this is the same cast responsible for that first awful dub of One Piece, it’s amazing what a talented director can squeeze out of them.Īnd then you’ve got writers! The first season features scripts from pros like Marty Isenberg, who wrote for Batman: The Animated Series and story edited Transformers Animated, and Eric Luke, who co-plotted the opening miniseries of Disney’s Gargoyles. And they actually bused in some top shelf talent that typically kept their distance from “The guys who took Yu-Gi-Oh and made it WORSE somehow”.Īlthough all the voice acting is strictly non-union, and the same people known for dubbing anime and dubbing it poorly, the voice direction was headed by none other than Susan Blu. When I sit back and ruminate on the “how” aspect, I try to look at the actual people involved in making the 4Kids TMNT cartoon and not just the production company logo. Listen to the latest episode of the AIPT Television podcast! ![]() This feels like the one of the main differences between the two games, and it’s nice to have an expanded dinosaur roster. Creating your first rare dinosaur from the DNA of excavated fossils still offers the same thrill as it did in the original, and JWE2 adds both flying and swimming dinosaurs, and the tasks that come along with looking after them as well. The game is largely similar to its predecessor, also benefiting and suffering from some of the same things. These levels should take you no more than a couple of hours at most, which seems a shame for the main campaign of a game, even if there are other modes to keep you occupied. It’s a good jumping off point, especially if you haven’t played the original JWE. The short campaign mode, surprisingly consisting of only five levels, takes you through most of the basics of the game, as you learn how to build your facility and manage everyone and everything within it. Being able to drive round and capture dinosaurs in the wild offers a new and interesting challenge, providing you with more exploration, albeit only slightly more as this wild area the dinosaurs roam is quite limited. You must work in unison with the DFW (Department of Fish and Wildlife) in order to get these dinosaurs under control and into a safer environment, namely a park that you’ll need to build from the ground up. JWE2 is set after the last Jurassic World film so, spoiler alert, dinosaurs are roaming free around America. With the popularity of dinosaurs never seeming to waiver, and with the third entry in the Jurassic World film trilogy on its way next year, what better time to bring out a sequel in the form of Jurassic World Evolution 2. If you grew up in a time when Jurassic Park was at the height of its popularity, and simulation games such as Theme Park World, Rollercoaster Tycoon and Sim City were making waves in the charts, the original Jurassic World Evolution was a must buy. We could use it in Firefox, Safari and Chrome as well. You really do get your money's worth.Ĭons: SplashID Safe works as a browser plug-in too, for automated login and password storage, but only in Internet Explorer. ![]() SplashData has been around since 2000 though, and in the software utility business, that's a very good track record. All of these sorts of utilities are competing heavily against each other so prices are low. What do you want for US$9.95? Considering the amount of minor aggravation and stress that SplashID Safe can alleviate, and considering that with SplashID Safe you can make an important and substantial improvement in your personal information security in every part of your life, this utility is dirt cheap. Examples of those passwords include the person's own name, password, the name of one of their children, and so on in that simple vein. The complain was largely the same from all of the testers: too many passwords to remember, too many different personal logins (from business intranets to Facebook, Twitter, Pinterest and you-name-it, to business and personal banking logins, etc.), and the really dangerous habit of using the login name or user name and password for almost everything. None of the people we drafted to try out SplashID were using any sort of serious password security of any kind. We used SplashID Safe, for the purposes of this review, in several different business, home and travel situations. But who wants to start memorizing a series of different and very long password phrases? See what I'm getting at? No matter how you analyze the data security situation, using a single long-ish password to secure SplashID is all you need to keep a secure hold on literally dozens, hundred or thousands of other highly complex passwords without having to personally remember even a single one of them. Why is the plain language password better? Because it's far longer, because it takes for more computing power to crack, far more time to crack because of the length, and most attempted automated botnet cracks will simply time out after a while and look for targets that yield results faster. This password is vastly better: "keepyourhandsoffmypersonalinfoyounastyvillain" It's a combination of numbers, upper and lower case letters, and a 'dash' symbol. "Gbv1-29dcF" is a perfectly good password. Where you were previously using the same user name and relatively simple password over and over again, it will dawn on you that without the need to remember a complex password you can dramatically improve your personal information and access security. The longer you use SplashID Safe, the less often you'll feel any need to simplify new login names and passwords for personal data sites (banks, trusts, financial management sites, etc.), software (online and desktop/laptop/mobile installations), news, entertainment and shopping web sites. SplashID Safe has a subtle but persistently positive effect on personal security. ![]() SplashID Safe can of course be used to store any kind of private data - passport numbers, bank account numbers, credit card numbers/expiry dates/security codes, travel insurance account numbers and the associated emergency phone numbers, and all sorts of other things. ![]() Synchronize data between the desktop software and a handheld client for multiple platforms - iPhone, iPad, Android, Blackberry, Palm OS, and Windows Mobile.Unbreakable AES and 256-bit Blowfish encryption provides proven protection for safe password management secure field masking offers additional protection for sensitive data like passwords - that means you can set up the password field to show only asterisks in case you find yourself in situations where someone next to you (or a security camera) might be able to see something) automatic password generator feature creates un-guessable passwords and prevents the security risk of using the same passwords repeatedly.Definable list views, support for custom icons and field labels.Unlimited number of customizable record types and categories for storing all kinds of passwords and personal information.Aside from that caveat, WiFi sync is fast and accurate. WiFi synchronization of mobile devices with your desktop installation works well, although you'd be wise to ensure your wireless network password is properly secure and additionally 'masked' by a unique station ID (SSID) for your business or home network wireless router. Scan the films at high enough DPI t avoid pixelated bands, or capture a high resolution exposure for IR imaging methods. It greatly improves consistency.ĭevelop your blots with similar times according to your dilution curve or empirical experience, as above. This normalizes the current for membrane area and transfer time. Load equal sample masses and transfer with constant current, using same mW*hr/cm 2 of membrane to deposit similar amounts of protein onto your membranes. It starts with the PAGE/transfer process. Here's how I do it when I need to quantify bands for biological replicates that are run on different membranes. It's not even worth quantifying saturated bands. Then establish/optimize the antibody concentrations, wash times/buffers, and development time.Īt a bare minimum make sure that you haven't saturated your signal (a max/min pixel value for your LUTs on imageJ, for example). So a good example for a mini gel would be 25ug lysate, 12.5ug, 6.25, 3.125, etc. This can be as simple as loading a serial dilution series of your lysate and then identifying an exposure range for film or IR detection that gives you measurable, non-saturated intensities for dilutions you usually load. Preface: It's best to find your linear range before even attempting quantification. Quantification is always the way to go, so you're on the right path. You want to be able to say that treatment led to a 2 or 3-fold change in fluroescence when you write it up, rather than just give a qualitative statement. You may not have to actually show it in the final figure, but just have it for yourself or for reviewers. I cannot recommend it enough if you have access, and if you do I would be happy to tell you all about it.Īs for whether or not this analysis is necessary, I would say that it is. ![]() Whenever I want to quantify protein that is at least somewhat abundant, I use fluorescent western blots with the LICOR Odyssey system. This is the guide I used in the past for ImageJ and chemiluminescent blots This is a reason I moved away from chemiluminescence to fluorescence, especially for for quantification.Ģ - Im wondering if you did something different in the analysis. Two things:ġ - The more intense bands look saturated, you should try a shorter exposure for quantification. The difference between those bands looks SO much bigger than D7. First thing I noticed - Unless I'm reading this incorrectly, there is NO way D3 is only a 2-fold change (or maybe its closer to 3-fold, but its hard to tell with how you've presented the data). ![]()
![]() The last time the Earth experienced a comparable concentration of CO2 was 3-5 million years ago, when the temperature was 2-3C warmer and sea level was 10-20m higher than now. He says: “CO2 remains in the atmosphere for centuries and in the ocean for even longer. However, it does provide us with a platform for more sustained and ambitious climate action to reduce emissions to net-zero through a complete transformation of our industrial, energy and transport systems.” The Independent also reports comments from Taalas. We need a sustained flattening of the curve.” He adds that “the Covid-19 pandemic is not a solution for climate change. BBC News reports that “CO2 will continue to increase this year, but that rise will be reduced by 0.08 to 0.23ppm” and that this value “falls within the 1ppm natural variability that occurs from year to year.” The WMO secretary-general Petteri Taalas tells the outlet that “the lockdown-related fall in emissions is just a tiny blip on the long-term graph. However, there is still a clear increase in CO2 levels measured this year when compared to those measured last year – the average CO2 level in the atmosphere this September was 411.3ppm, “up from 408.5ppm in September 2019”. The Guardian says that “there is estimated to have been a cut in emissions of between 4.2% and 7.5% in 2020 due to the shutdown of travel and other activities”. There is widespread media coverage of the annual greenhouse gas bulletin released by the World Meteorological Organization (WMO) yesterday which states that, despite the global coronavirus lockdowns, levels of CO2 in the atmosphere have reached a new record high. The Wall Street Journal , Axios, MailOnline and the Independent also report on this story, whilst Mother Jones runs a story on “seven ways that Biden can fight climate change without any help from Congress”. It reports that he “met president Xi Jinping in 2014 during a six-day visit to advance collaboration on climate action between the world’s two largest emitters.” The New York Times reports that “those who have worked with him describe Mr Kerry as having real understanding of the science of climate change, a grasp of the economic costs and benefits of moving to clean energy and a close working relationship with dozens of leaders in the field.” Reuters also reports on this story, adding that Biden has named former Federal Reserve chair Janet Yellen as US Treasury secretary. It adds that “Kerry will report directly to Biden as part the White House’s National Security Council, underscoring how the incoming administration views the warming planet as an issue with implications for both national defense and foreign policy.” The Hill and Climate Home News refer to Kerry as a “climate czar”, with the latter highlighting Kerry’s work with the Chinese government. The Washington Post reports that Kerry has spent much of his career working to reduce the impacts of climate change and that he “has represented the United States at every pivotal international climate conference in the past three decades”. ![]() The newpaper also reports that Kerry was part of a “climate taskforce the Biden campaign used to develop its carbon-cutting policies” over the summer, and that, according to the Biden transition team, “this elevation shows the president-elect sees the climate crisis as an ‘urgent national security issue’”. The Guardian reports that since leaving government in 2017 “Kerry has been sharply critical of Donald Trump’s dismantling of climate policies and the decision to remove the US from the Paris agreement”, adding that the Biden team plans to reenter the Paris Agreement. The news comes as the Trump administration finally gives the “green light” for government officials to proceed with the Biden transition, according to Reuters. Kerry “served as secretary of state during Barak Obama’s second term as president” and is now set to be the “first official dedicated to climate change to sit on the National Security Council.” The outlet reports that “Kerry signed the Paris climate agreement on behalf of the US in 2016” and that in 2019 he launched “World War Zero” – a “coalition of world leaders and celebrities” who called for net-zero carbon emissions and action on climate change. Former US secretary of state John Kerry will act as climate envoy when US president-elect Joe Biden takes office in January, the BBC reports. Adults and kids alike can cool off in the calm lagoon pools or swim in the ocean protected by marine stinger nets.īiking, jogging, and strolling along the promenade are other popular things to do on The Strand, and you can also fish off the jetty, tone on the outdoor fitness equipment, and kayak or paddleboard in the calm waters. Coconut palms sway in the breeze, the blue-green waters of the bay beckon beyond the golden-sand beach, and Magnetic Island slouches lazily on the horizon.Ĭhildren love the playgrounds and the colorful Strand Water Park, where tipping buckets, mini slides, and sprinklers spritz them on hot days. This is a great spot to soak up the tropical vibe when you first arrive in town. Stretching for 2.5 kilometers along the calm waters of Cleveland Bay, The Strand is a popular beachfront promenade and a magnet for both tourists and locals. Start planning your tropical getaway with our list of things to do in Townsville. Other popular things to do in Townsville include diving one of the world's best wreck dives, exploring marine life at the world-class aquarium, and wandering through the city's beautiful parks and botanical gardens. From here, you can hop aboard a cruise to the World Heritage-listed Great Barrier Reef or take a 20-minute ferry ride to Magnetic Island, with its beautiful bays, bushland hikes, and wild koalas.īehind the city, 290-meter-high Castle Hill, a pink granite monolith, provides a panoramic lookout for both locals and tourists who love to hike to its peak. On the pink-tinged headland at one end of this popular walking path, the fascinating outdoor exhibits of Jezzine Barracks trace Townsville's rich military history and Aboriginal heritage.Īt the other end of the Strand, Ross Creek is home to the city's port. The Strand, a palm-lined promenade skirting Cleveland Bay and its golden-sand beach, is the city's pride and joy. In the town center, high-rises hint at its role as the de facto capital of the region, but it still manages to pull off plenty of touristy charm. We may earn a commission from affiliate links ( )ĭown-to-earth Townsville, in the dry tropics of North Queensland, is the largest tropical town in Australia and basks in more than 320 days of sunshine a year. ![]() Continued abuse of our services will cause your IP address to be blocked indefinitely.Written by Karen Hastings Updated Sep 13, 2021 Please fill out the CAPTCHA below and then click the button to indicate that you agree to these terms. If you wish to be unblocked, you must agree that you will take immediate steps to rectify this issue. If you do not understand what is causing this behavior, please contact us here. If you promise to stop (by clicking the Agree button below), we'll unblock your connection for now, but we will immediately re-block it if we detect additional bad behavior. 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